Tyrone, Pablo and Tasha are the world-famous band Los Galacticos. After receiving a new tour bus, they learn that it's really a spaceship! After accidentally launching themselves into space, they learn that Earth is in danger from Gooniqua and Goostin, so they decide to defend the Earth, even though they don't know how to fly the ship.
Pablo, Tyrone, and Tasha are in the backyard wearing black and white sombreros and holding instruments. They introduce themselves as the members of the world-famous band Los Galacticos. They sing Los Galacticos. Tyrone explains how they are recieving a new tour bus. The backyard transforms into a mostly-empty parking lot. The band thinks they have spotted their new tour bus and get inside the "bus". Pablo tells the others that it does not look like a normal tour bus.
Tyrone activates the vehicle. It flies off, revealing their real tour bus behind it. The band notices that they are flying and realize that they are not in a tour bus. They are in a spaceship. The ship pilots them into outerspace. The three band members are petrified.
Meanwhile, two Googors (aliens), Gooniqua and Goostin, are searching space for objects to cover in goo. They sing We Love Goo as they explain how they love "gooing" things. They spot Pablo, Tyrone and Tasha in the spaceship and goo it. The window is covered in goo. Tyrone uses the windshield wipers to wipe the goo off. Gooniqua and Goostin see them driving toward them and think they are trying to stop them. Gooniqua sends the band a video of her telling them that they will goo their planet Earth.
The band is confused. Tyrone tells the others that if they are going to save Earth, they must learn to control the spaceship and stop the Googors. They sing Pushing Every Button as they find out what every button and control in the ship does. The aliens see the ship spinning around and think the band is retreating. They laugh and goo a meteor.
The three band members are satisfied after they have learned what every button does. They tell the Googors to "bring it on". The Googors try to goo them, but Tyrone dodges every shot. After shooting a 'goo pie' at the band members, Goostin and Gooniqua prepare a giant goo bubble to be launched toward planet Earth. After shaking the goo off their spaceship, Tyrone accelerates the ship's speed and commands the band to turn the ship, after passing the goo bubble. Pablo activates the shield, and the goo bubble rebounds off the spaceship, and heads straight toward the Googors' ship. It splats on the ship, making it spin out of control. Goostin and Gooniqua scream. Before their ship crashes into a nearby moon, Tyrone teleports them out of their ship and into theirs.
The Googors thank Los Galacticos and are brought to Earth. The five sing Earth Es Muy Bueno as they describe how wonderful Earth is. Tyrone compliments the Googors' singing, and offers them to join their Mariachi band. Goostin and Gooniqua accept. Goostin then notices the real Los Galacticos tour bus and asks about it. "Wait a minute... If this is your bus, whose is that?" The band members suddenly realize that they boarded the wrong bus. Three female aliens appear and ask Los Galacticos for their autograph. They see the ship that the band piloted and enter it. They wave goodbye and zip into space.
Tyrone's stomach growls. Pablo asks if the sound came from another spaceship. Tyrone tells him it was just his stomach and invites everyone over for chips and salsa. The characters sing the end song and run into Tyrone's house for a snack. Pablo, Tyrone, and Tasha open the previously closed door and shout "Los Galacticos!".