Cowgirl Uniqua is a highly competitive horse rider, known for winning races. She owns a horse named Lightning. She and Jockey Pablo once entered a race with Farmer Tyrone by the Mississippi River. During this race, Uniqua Pablo frequently bickered and competed with each other in different activities to find out who is superior. Their valiant efforts in each sidequest proved that their abilities are equal.
Cowgirl Unqiua is mostly pink. She wears a light brown cowboy hat and a purple shirt, tan pants, a black belt, white boots patterned with pink flowers, and a red bandana.
Uniqua has played Cowgirl Uniqua in one episode of The Backyardigans: "Horsing Around" from season two.